As in all biology, good protocols evolve over time, but printed copies often don’t. Here are my version-controlled write-ups:

Identifier	Title	Domain	Status	Last revision
Chapter .1	Basics		Internal	2025-05-07
SOP0001.2	Isolation of nuclei from tissue culture cells	Cell biology	Public	2026-05-22
SOP0002.2	Whole cell lysates from tissue culture cells	Cell biology	Public	2026-09-07
SOP0003.1	Native polyacrylamide gel electrophoresis	Biochemistry	Public	2025-03-30
SOP0004.3	Core histone expression and purification	Biochemistry	Internal	2026-07-31
SOP0005.2	Histone octamer, tetramer, or dimer formation	Biochemistry	Public	2025-03-27
SOP0006.3	Reconstitution of mononucleosomes and nucleosome arrays	Biochemistry	Internal	2026-05-10
SOP0007.1	Denaturing SDS polyacrylamide gel electrophoresis	Biochemistry	Public	2024-07-17
SOP0008.1	Western blotting	Biochemistry	Public	2026-05-22
SOP0009.1	Immunoblotting	Biochemistry	Public	2026-05-22
SOP0010.1	In-gel protein staining with Coomassie Brilliant Blue	Biochemistry	Public	2023-01-16
SOP0011.1	In-gel protein staining with silver nitrate	Biochemistry	Public	2026-05-22
SOP0012.2	Non-viral transfection of animal tissue culture cells	Tissue culture	Public	2026-06-11
SOP0013.1	Electroporation of animal tissue culture cells	Tissue culture	Internal	2023-03-09
SOP0014.1	Counting cells	Tissue culture	Public	2023-03-14
SOP0015.1	Cultivating HeLa cells	Tissue culture	Public	2023-03-13
SOP0016.1	Cultivating MCF 10A cells	Tissue culture	Public	2023-03-14
SOP0017.3	Lentiviral generation of stable animal cell lines	Tissue culture	Public	2026-05-22
SOP0018.1	Generation of stable cell lines using DNA transposases	Tissue culture	Public	2023-11-12
SOP0019.1	Selection and screening of stable mammalian cell lines	Tissue culture	Public	2023-04-28
SOP0020.1	Plasmid preparation by alkaline lysis	Molecular biology	Public	2026-05-21
SOP0021.1	Purification of nucleic acids	Molecular biology	Public	2023-06-10
SOP0022.1	Purification of nucleic acids from agarose gels	Molecular biology	Public	2026-05-21
SOP0023.1	Separation of RNA from DNA with guanidinium thiocyanate	Molecular biology	Public	2023-06-10
SOP0024.1	Extraction of genomic DNA from tissue culture cells	Molecular biology	Public	2023-11-11
SOP0025.2	Nuclear extraction and fractionation of chromatin-associated proteins	Cell biology	Public	2026-09-07
SOP0026.1	Acidic extraction of histone proteins	Cell biology	Public	2023-10-26
SOP0027.2	Immunoprecipitation of soluble proteins	Biochemistry	Internal	2026-09-07
SOP0028.2	Cell cycle synchronization of mammalian cell lines	Tissue culture	Internal	2025-04-17
SOP0029.1	Cell cycle analysis by fluorescence staining	Tissue culture	Internal	2026-07-31
SOP0030.1	Making reagents and buffers	Core skills	Public	2026-02-25
SOP0031.1	Designing and performing dilutions	Core skills	Public	2026-02-26
SOP0032.1	Making and running agarose gels	Molecular biology	Public	2026-05-22
SOP0033.1	Restriction digest, end modification, and ligation of DNA	Molecular biology	Public	2024-07-22
SOP0034.1	Transformation of plasmids into a bacterial host	Molecular biology	Public	2026-06-11
SOP0035.2	Writing a good protocol	Core skills	Public	2026-05-12
SOP0036.2	Banking, tracking, and sharing of materials	Core skills	Public	2026-05-13
SOP0038.1	Design and cloning of CRISPR guide RNAs	Tissue culture	Public	2025-05-28
SOP0039.1	Qualitative polymerase chain reactions	Molecular biology	Public	2026-05-22
SOP0040.1	In vitro transcription with T7 RNA polymerase	Molecular biology;biochemistry	Internal	2026-05-22
SOP0042.1	Environment management for scientific computing	Data management	Public	2026-05-10
SOP0043.1	Project layout and reproducibility for R analyses	Data management	Internal	2026-05-16
SOP0044.1	TEV protease production and use as a cleavage tool	Biochemistry;molecular biology	Internal	2026-05-29
SOP0045.1	IMAC purification of His-tagged proteins	Biochemistry;molecular biology	Internal	2026-06-01
SOP0101.2	Version control using Git and GitHub	Data management	Public	2026-05-12

Protocols