Protocols

Good protocols evolve over time, but printed copies often don’t. Here, I share version-controlled write-ups to help track changes. You can check whether your printed copy is current, or just browse for the latest method:

Identifier Title Domain Status Last commit
SOP0001.1 Isolation of nuclei from tissue culture cells Cell biology Public 2025-11-03
SOP0002.1 Whole cell lysates from tissue culture cells Cell biology Internal
SOP0003.1 Native polyacrylamide gel electrophoresis Biochemistry Internal
SOP0004.3 Core histone expression and purification Biochemistry Internal
SOP0005.2 Histone octamer, tetramer, or dimer formation Biochemistry Internal
SOP0006.2 Reconstitution of mononucleosomes and nucleosome arrays Biochemistry Internal
SOP0007.1 Denaturating SDS polyacrylamide gel electrophoresis Biochemistry Internal
SOP0008.1 Western blotting Biochemistry Internal
SOP0009.1 Immunoblotting Biochemistry Internal
SOP0010.1 In-gel protein staining with Coomassie Brilliant Blue Biochemistry Internal
SOP0011.1 In-gel protein staining with silver nitrate Biochemistry Internal
SOP0012.2 Non-viral transfection of animal tissue culture cells Tissue culture Internal
SOP0013.1 Electroporation of animal tissue culture cells Tissue culture Internal
SOP0014.1 Counting cells Tissue culture Internal
SOP0015.1 Cultivating HeLa cells Tissue culture Internal
SOP0016.1 Cultivating MCF 10A cells Tissue culture Internal 2025-10-25
SOP0017.3 Lentiviral generation of stable animal cell lines Tissue culture Internal
SOP0018.1 Generation of stable cell lines using DNA transposases Tissue culture Internal
SOP0019.1 Selection and screening of stable mammalian cell lines Tissue culture Internal
SOP0020.1 Plasmid preparation by alkaline lysis Molecular biology Internal
SOP0021.1 Purification of nucleic acids Molecular biology Internal
SOP0022.1 Purification of nucleic acids from agarose gels Molecular biology Internal
SOP0023.1 Separation of RNA from DNA with guanidinium thiocyanate Molecular biology Internal
SOP0024.1 Extraction of genomic DNA from tissue culture cells Molecular biology Internal
SOP0025.1 Nuclear extraction and fractionation of chromatin-associated proteins Cell biology Internal
SOP0026.1 Acidic extraction of histone proteins Cell biology Internal
SOP0027.1 Immunoprecipitation of nuclear and cytosolic proteins Biochemistry Internal
SOP0028.2 Cell cycle synchronization of mammalian cell lines Tissue culture Internal
SOP0029.1 Cell cycle analysis by fluorescence staining Tissue culture Internal
SOP0030.1 Making reagents and buffers Core skills Public 2025-11-03
SOP0031.1 Designing and performing dilutions Core skills Internal 2025-11-03
SOP0032.1 Making and running agarose gels Molecular biology Internal
SOP0033.1 Restriction digest, end modification, and ligation of DNA Molecular biology Internal
SOP0034.1 Transformation of plasmids into a bacterial host Molecular biology Internal
SOP0035.1 Writing a good protocol Core skills Internal 2025-11-03
SOP0036.1 Banking, tracking, and sharing of materials Core skills Internal 2025-11-03
SOP0037.1 Quantitative amplicon sequencing with unique molecular identifiers Molecular biology Draft
SOP0038.1 Design and cloning of CRISPR guide RNAs Tissue culture Internal
SOP0039.1 Qualitative polymerase chain reactions Molecular biology Internal
SOP0101.1 Version control using Git and GitHub Data management Public 2025-11-03
🚧 This table is still being updated Buy me a coffee · GitHub: (not yet public)